Sharp zoom lens coupled to a zero defect, Sony scientific grade CCD or CMOS Geldocumentation system, is a high quality, yet low cost professional gel and bio-imaging system. Options fromagarose, PAGE, western blots, dot blots, nucleic acid, colorimetric to fluorescent & chemifluorescent imaging,Colonies, TLC, ELISA Plates, Petri Dish etc. and upgradeable to chemiluminescence.
Featuring high resolution extremely sensitive 16-bit scientific grade CCD camera (with choice of 1.4, 5.6, 6.3 and 9 megapixel cameras) with extremely low noise and dynamic flat field calibration coupled to a C-mountmotorized zoom lens (with option for numerical feedback value) that is pre-focussed for different zooms orsample setting, mounted inside an enclosure that covers and protects the camera and lens. The camera, lens &cabinet connects to & can be controlled from the PC using live-view acquisition capture software.
The mild & stainless steel, epoxy coated microprocessor controlled dark room cabinet has features for UV irradiation safety switch that switches off the UV lights as soon as the door is opened. Additionally, a timer is provided that switches off UV/White light in case the user forgets to shut it off. Option to adjust timer for excision. Also, the user can opt for automatic switching on of the Dual Epi-White LEDs on opening of door and for automatic controls of UV light and exposure. A transilluminator cooling system is also provided to prevent overheating of gels. Option for a front viewing window (with amber filter) & 2 side access doors for gel observation & cutting. Gel platform temperature control (<34 C) & alignment templates also available. Illumination Choice of illumination options with UV trans/epi lighting, wavelength-specific LEDs and more, add to theversatility of the system. UV roll-out transilluminator can be provided in dual 302-312nm (detection range downto 0.1 ng with EtBr, SYBR etc. & 10 pg with Vistra Green) and 365nm (additionally 254nm, for triple wavelengthconfiguration), and even Red, Green, Blue LEDs ranges. Large filter size of transilluminator for gel images up to26x26cm, with extra large special filter size of up to 28x38cm is also available. Colorimetric samples up to38x30cm can be captured. Epi-white light illumination from above is standard using two LED tubes, with anoption for low (50%) & high (100%) intensity control. Optionally, ultraviolet epi-illumination in a variety ofwavelengths (254/312/365nm) can also be provided. Standard Trans White light and Trans Blue light tables upto25x25 cm are also available with options for sizes up to 30 x 37 cm. # Please note that 300nm, 302nm, 306nm and 312nm are in the same range of wavelengths called UV-B (also known as mid-range). Some manufacturers quote 302nm but it is the same as 312nm.. Epillumination Epi-white light illumination through dual LED tubes from above is standard. Ultraviolet epi-illumination in a variety of wavelengths can also be provided. For specific stains, epi-light in blue, green or red LEDs can be provided. A combination of all these illumination modes in the same system can be provided. Filters * Orange 590: Ethidium Bromide (EtBr), SYPRO Ruby, ProQ Diamond, Deep Purple, etc * Green 520: SYBR Green I, Green II, SYBR Gold, GFP, Fluorescein, FITC * Orange 560: SYBR Safe, Rhodamine, Cy3, etc * Red 630: SYPRO Red, Texas Red, etc * Blue 480: Hoechst, Coumarin, etc * Clear: Coomassie Blue, Silver etc Filter Holder * Standard is a manual 3-position filter holder * Advanced models with 3, 4 and 7-position manual and motorized filter holder are also available * Orange 590 EtBr and clear filters are standard. How the Gel Documentation & Imaging Analysis System works A transilluminator is mounted on roll-out rails inside a light-tight cabinet. To place the gel, the door is opened and transilluminator pulled out. Push it in, close the door and switch on the UV light and camera. The camera is mounted on top of the cabinet along with zoom lens and wavelenght filter (nominally 590nm filter suitable for Ethidium Bromide EtBr gels is provided). Connect the camera to the PC using the USB cable and gel capture software, to get a real-time live view image of the gel. Adjust camera settings (like shutter speed, aperture, focus) till the optimum image is obtained. Click your images and save them on the PC. A white light epi-illuminations source is also provided, that illuminates objects from top. With the optional 1D gel analysis software, you can analyse the gels. Package Includes Dark room cabinet with safety switch with low footprint Roll out variable intensity 312nm transilluminator Epi-white light illumination CCD camera Gel capture software 590nm orange filter, suitable for EtBr stained gels Power cords, USB cable, instruction manual, warranty card Options that can be ordered like motorized filter holder, special zoom lens, illumination etc Gel Capture Software Gel image acquisition and control software has full function control panel which allows user to adjust camera, lens settings like exposure time settings, motorized zoom, focus and aperture (iris). Histogram display on the screen shows image saturation and indicates over-saturation/exposure. Options for manual exposure, auto exposure and exposure for intense & faint bands are available. Application protocols can be further edited/created and saved with specific controls for light source, camera settings, exposures, aperture etc. The user can opt to choose the manual mode or automatic mode of light source, focus, exposure & aperture. Option for Manual or Motorized filter wheel with Triple, 4 or 7 filter slots are available. Optional :- Workflows to suit your analysis requirements Gel analysis is rapid, automated to a high level and 100% reproducible and repeatable using application specific/user-defined protocols. The user has the ability to review each stage of the workflow analysis and intervene / edit if required. Combining high levels of automation with final user review allows rapid and accurate quantitative analysis. The user then has full control of the visualization tools and data display - outputting only those data fields that are of importance as well as the images of choice. Thus results are completely reproducible and repeatable. Fast Accurate Quantitation and Reporting Advanced colorimetery & densitometry software for 1D gels, colonies, dot blots, arrays, multiplex images and RFLP analysis, highly developed algorithms accurately detect lanes and bands even on distorted gel images. Results can be verified using the range of visualisation tools which aid further examination of lane and band data. Calibrate the bands using one or more Molecular Size standard lanes and derive accurate quantitation from known band volumes. Analysis and data can be exported as Excel files and pdf files. Image files can be saved and exported in jpg, png, tiff and bmp formats, with an option for password protection . Also provided is a 3D viewer for viewing bands in 3D. Options available for upgradation of tools compliant with US FDA 21 CFR Part 11 regulations. General Image Analysis Toolbox, Array Analysis, Dot Blot and Colony Counting Modules Define circular or rectangular area of interest. Easy control slider bars for sensitivity and operator size. Initialise sensitivity option. Advanced parameter settings for sensitivity, noise, operator size and background. Automatic Splitting function. Total count and spot data automatically displayed. Wide range of data fields to display in measurements table. Edit, draw, erase, delete or split features. Adjustable pen size for drawing or erasing. Renumber features. GENERAL * Fully automatic, single button press imageacquisition as final resultant image * Fully automatic, single button press completeimage analysis within area of interest * Edit and enhance image for improvinganalysis or create multiplex images * Brightness, contrast, annotate, illustrate or crop images & export for copy paste function * Instant access to refinement of analysis step * Alternative step-wise image analysis * Facility to load and save user preferences,including parameters and display options,prior to analysis * Automatic PDF report generator * Ruler options to display lane names, numbersand Moleuclar weights * Rf and IEF propagation distance calculation * Single button press export option forpublication ready images. * Print image as per user’s choice of dpi BACKGROUND SUBTRACTION * Automatic methods * Rolling ball, Rubber band, Minimum profile, Valley to valley, Lane edge subtract * Manual methods BAND DETECTION * Fully automatic multiple band detection * Adjustable peak parameters: * Minimum peak * Noise reduction * % max peak of lane or gel * Band separation detection methods: * Automatic separation from background * Single Edge * Fixed width * Volume and Intensity of bands displayed * Manual editing of peak and edge detection in image and lane profile windows * Snap to peak editing * Automatic band measurements * View band measurements in measurements table * Wide range of data fields to display in measurements table * Histograms for viewing band data * View multiple lane profiles stacked/overlaid * Export lane profile information * Edit Band Name and display on image * Automatic Dendrogram creation * New noise reduction option for band detection removes dust from image * Median filter LANE CREATION * Automatic multiple lane detection * Export and import of lane templates * Manual lane detection * Multi-tier analysis * Move, resize and bend multi-box * Move, resize, bend individual lanes * Add grimaces to account for band distortion * Delete lanes PROFILE DECONVOLUTION * Fit Gaussian curves to profile * One Gaussian per band * Manual adjustments of Gaussian * FWHM (Full Width Half Max) measurement of bands MOLECULAR WEIGHT/ Rf CALIBRATION * Calibrate, detect and calculate Molecular Weights of 1D gels * Library of standards * Add and Edit existing standards * Automatic assignment of standard bands * Propagation by Rf between standards * 6 curve fitting methods * Molecular Weights automatically displayed * pI standards can be increased or decreased * Volume and Intensity displayed